By Thomas Rabe, Ludwig Kiesel, Benno Runnebaum (auth.), Dr. Detlev Ganten M.D., Ph.D., Dr. Donald Pfaff Ph.D. (eds.)
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Additional info for Actions of Progesterone on the Brain
Kiesel, and B. Runnebaum Beyer B, Terenius L, Brueggemeier RW, Ranade VV, Counsell RE (1976) Synthesis of potential antiprogestins. Steroids 27:123-131 Bouin P, Ancel P (1910) Sur les fonctions du corps jaune gestatif. 1. Sur Ie determinisme de la preparation de l'uterus ala fixation de l'oeuf. J Physiol Pathol Gen 12:1-16 Brown WE, Bradbury JT (1947) A study of the physiologic action of human chorionic hormone. Am J Obstet GynecoI53:749-757 Butenandt A, Westphal U, Hohlweg W (1934) Uber das Hormon des Corpus lutenum.
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All subsequent procedures, unless otherwise noted, were performed at 4 DC. Sucrose Density Gradient Centrifugation. 1 Ci/mmol] in the absence or presence of a 100-fold excess of unlabeled steroid hormones dissolved in 5 Jll ethanol. The mixture, layered on a sucrose-l0% glycerol density gradient, was centrifuged in a Beckman model L3-50 or a Hitachi RP65 ultracentrifuge as previously described (Kato and Onouchi 1977). 2-ml fractions) and counting was performed as previously described (Kato and Onouchi 1977).