By Akira Mitsui
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1974. Hydrogenase, photoreduction and anaerobic growth. In: A lga l Physiology and Biochemistry. P. Stewart. Blackwell S cien tific Publication, Oxford, p. 456. King, D. and M. Gibbs. 1976. Hydrogen metabolism in photo synthetic organisms: the role of carbon metabolism. A bstract of the Annual M eeting o f the Am erican Society of Plant Physiologists, p. 60. Kok, B. 1973. Photosynthesis. In: Proceedings o f the Workshop on Bio-Solar Conversion. (Ed) M. Gibbs, A . Hollaender, B. O. K ram pitz, and A .
We have chosen this "long term " method because it makes it possible to discuss the com m ercially important question o f stability and quantity of H2 production. Most previous experiments show that hydrogen photoproduction lasts only a very short tim e (a fe w minutes to 1 hour). In the first set o f experiments several strains o f blue-green algae were shown to produce H2 gas in the dark (see Table 1). Approxim ately one half o f the tested species produced hydrogen in the dark (59). The fa c t that so many strains o f tropical marine blue-green algae produce H~ in the dark coincides with the results o f B en-A m otz e t al.
A. Paneque and D. I. Arnon. 1962. Photoreduction of m eth ylviologen by isolated chloroplasts. (Manuscript) Photoproduction o f hydrogen by ch loroplast-m ethylviologenhydrogenase system. (Manuscript) Photoproduction o f hydrogen and c y c lic and n on-cyclic phosphorylation by chromatophores o f blue-green algae. Quoted in follow in g review papers by D. I. Arnon: Photosynthetic Mechanisms of Green Plants. (Eds) B. Kok and A . T. Jagendorf. Pub. No. 1145, Washington, D. C. N A S -N R C . p. 195.