Cancer and Zebrafish: Mechanisms, Techniques, and Models by David M. Langenau

By David M. Langenau

This quantity makes a speciality of defining the original attributes of utilizing the zebrafish melanoma version for locating vital pathways and power drug pursuits for the remedy of human cancers. utilizing the zebrafish version, the quantity explores oncogene and tumor suppressor discovery, chemical genetic methods, genomics, epigenetics, melanoma imaging, and cellphone transplantation. Contributed chapters come from the main trendy laboratories operating during this box, which supplies a special viewpoint on zebrafish types from a large spectrum of the study neighborhood. moreover, the e-book bargains a close research of the most up-tp-date study within the sector for particular zebrafish melanoma versions, together with T cellphone leukemia, rhabdomyosarcoma, liver and pancreatic melanoma, cancer, neuroblastoma, germ phone tumors, and malignant peripheral sheath tumors. A bankruptcy is additionally devoted to the improvement and usage of different piscine types of melanoma. The compilation of chapters within the quantity culminates right into a accomplished and definitive textual content on zebrafish and melanoma, supplying a miles wanted source at the strong attributes of the zebrafish version system.

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In different studies it was shown to reliably control gene expression in a dose dependent manner and without measurable leakiness [146, 147]. In a further evolution of the rag2 controlled leukemia model developed in the lab of T. Look [15] the cMyc oncogene is fused to the ligand binding domain of ER [147]. This allows for conditional activation of the oncogenic effect of cMyc through application of 4-OHT which leads to development of fully established T-ALL already after 5 weeks of treatment, while withdrawal of 4-OHT blocks cMyc activity and leads to apoptosis of cancer cells.

Few years later Liu et al. proved that the Cre/lox system can also be applied for gene inversion in zebrafish, although with very low efficiency [60]. Using the LE (left excision)/RE (right excision) mutant sites lox71 and lox66, respectively, they showed that a lox66 flanked RFP (red fluorescent protein) coding sequence can be integrated into a stable single-site lox71 line through co-injection of Cre mRNA and the vector pZklox66RFP. Shortly after, it was shown that—just as in mouse—Cre can be stably 30 M.

Mione bers would increase Gal4 efficiency but also leads to side effects [32]. Using GFP (green fluorescent protein) and the newly developed Gal4FF, Kawakami et al. [33] generated a large library of diverse gene trap and enhancer trap lines which are published and available to the zebrafish community under the name zTrap (see Box 1 for web sites). Distel et al. applied a third version of the VP16 AD, called TA4 [24]. It contains three copies of VP16436–447 but two of them with weakening mutations in the position 442, a position crucial for its function [34, 35].

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